Experimental Research Article

Korean J Pain 2022; 35(2): 140-151

Published online April 1, 2022 https://doi.org/10.3344/kjp.2022.35.2.140

Copyright © The Korean Pain Society.

Antinociceptive, anti-inflammatory, and cytotoxic properties of Origanum vulgare essential oil, rich with β-caryophyllene and β-caryophyllene oxide

Armenuhi Moghrovyan1 , Lilya Parseghyan2 , Gohar Sevoyan3 , Anna Darbinyan2 , Naira Sahakyan4 , Monica Gaboyan5 , Zaruhi Karabekian3 , Armen Voskanyan2

1Department of Pharmacognosy, Yerevan State Medical University after M. Heratsi, Yerevan, Armenia
2Orbeli Institute of Physiology, Laboratory of Physiologically Active Substances Investigations, Yerevan, Armenia
3Orbeli Institute of Physiology, Laboratory of Tissue Engineering, Yerevan, Armenia
4Yerevan State University, Research Institute of Biology, Faculty of Biology, Yerevan, Armenia
5Yerevan State Medical University after M. Heratsi, Faculty of Pharmacy, Yerevan, Armenia

Correspondence to:Armen Voskanyan
Orbeli Institute of Physiology, National Academy of Sciences, 22 Orbeli Bros. str. 0028 Yerevan, Armenia
Tel: +374 94818197
Fax: +37410 2802050
E-mail: arminvosking@gmail.com

Handling Editor: Jong Yeon Park

Received: November 23, 2021; Revised: February 1, 2022; Accepted: February 2, 2022

This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.


Background: Essential oils are of great interest for their analgesic and anti-inflammatory properties. We aimed to study the content of the essential oil of the Origanum vulgare of the Armenian highlands (OVA) in different periods of vegetation and to investigate its antinociceptive and anti-inflammatory effects in mice (in vivo) and cytotoxic action in cultured cells (in vitro). OVA essential oil was extracted from fresh plant material by hydro-distillation.
Methods: For OVA essential oil contents determination the gas chromatography-mass spectrometry method was used. Formalin and hot plate tests and analysis of cell viability using the methyl-thiazolyl-tetrazolium (MTT) assay were used.
Results: The maximal content of β-caryophyllene and β-caryophyllene oxide in OVA essential oil was revealed in the period of blossoming (8.18% and 13.36%, correspondently). In the formalin test, 4% OVA essential oil solution (3.5 mg/mouse) exerts significant antinociceptive and anti-inflammatory effects (P = 0.003). MTT assay shows approximately 60% cytotoxicity in HeLa and Vero cells for 2.0 μL/mL OVA essential oil in media.
Conclusions: The wild oregano herb of Armenian highlands, harvested in the blossoming period, may be considered as a valuable source for developing pain-relieving preparations.

Keywords: Analgesia, Anti-Inflammatory Agents, Beta-Caryophyllene, Caryophyllene Oxide, Cell Survival, Gas Chromatography-Mass Spectrometry, Nociception, Oils, Volatile, Origanum, Pain, Pain Measurement.