Fig. 6. Down-regulation of DUSP1 inhibited the alleviating effect of down-regulation of miR-382-5p on neuropathic pain. (A) The mRNA level of DUSP1 in DRGs was detected by RT-qPCR on the day 21 of CCI surgery. (B, C) Mechanical nociceptive sensitivity and thermal nociceptive sensitivity were measured in CCI rats on days 0, 3, 7, 14 and 21 of the CCI surgery. (D–F) On day 21 of CCI surgery, the levels of proinflammatory factors (IL-6, IL-1β, TNF-α) in DRGs were measured by ELISA assay. Values are presented as mean ± standard deviation. DUSP1: dual specificity phosphatase-1, DRGs: dorsal root ganglions, RT-qPCR: real-time quantitative reverse transcription polymerase chain reaction, CCI: chronic constriction injury, IL-6: interleukin-6, IL-1β: interleukin-1β, TNF-α: tumor necrosis factor-α, ELISA: enzyme-linked immunosorbent assay, PWT: paw withdrawal threshold, PWL: paw withdrawal latency. ###P < 0.001 vs. CCI + LV-anti-NC. &&P < 0.01; &&&P < 0.001 vs. CCI + LV-anti-miR-382-5p + sh-NC. n = 13.
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