Fig. 5. DUSP1 is a target gene of miR-382-5p. (A) There are complementary binding sites between miR-382-5p and DUSP1. (B) DLR assay confirms the targeting relationship between miR-382-5p and DUSP1. (C) RIP assay shows that both miR-382-5p and DUSP1 could be enriched in immunoprecipitation complexes. (D–F) The mRNA and protein levels of DUSP1 in DRGs were measured by RT-qPCR and ELISA on days 0, 3, 7, 14 and 21 of the CCI surgery. (G) On day 21 of CCI surgery, RT-qPCR was used to detect the effect of lentivirus interfering vector (LV-anti-miR-382-5p) on DUSP1 mRNA levels in DRGs. Values are presented as mean ± standard deviation. DUSP1: dual specificity phosphatase-1, GAPDH: glyceraldehyde 3-phosphate dehydrogenase, DLR: dual-luciferase reporter, RIP: RNA immunoprecipitation, DRGs: dorsal root ganglions, RT-qPCR: real-time quantitative reverse transcription polymerase chain reaction, ELISA: enzyme-linked immunosorbent assay, CCI: chronic constriction injury, WT: wild type, MUT: mutant. *P < 0.05; **P < 0.01; ***P < 0.001 vs. Sham group. ###P < 0.001 vs. CCI + LV-anti-NC. n = 13.
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