Primer sequence of RT-qPCR
| Gene | Primer sequence (5'-3') | |
|---|---|---|
| MiR-382-5p | F: TCATTCACGGACAACACTTTT | |
| R: GTGTCGTCGAGTCGGCAATTC | ||
| DUSP1 | F: ATATCGTGCCGAACACCCGAA | |
| R: ACGCTTCATATCCTCCTTGG | ||
| U6 | F: GCTTCGGCAGCACATATACTAA | |
| R: AACGCTTCACGAATTTGCGT | ||
| GAPDH | F: GTCGGTGTGAACGGATTT | |
| R: ACTCCACGACGTACTCAGC | ||
RT-qPCR: real-time quantitative reverse transcription polymerase chain reaction, DUSP1: dual specificity phosphatase-1, U6: U6 small nuclear RNA, GAPDH: glyceraldehyde 3-phosphate dehydrogenase.