Effect of APE from Trigona thoracica on the hot plate test in male mice

Treatment Dose (mg/kg) Latency of discomfort (sec) at respective time interval (min)
0 min 60 min 90 min 120 min 150 min 180 min 210 min
Normal saline - 4.2 ± 0.4 4.8 ± 0.2 5.1 ± 0.3 4.3 ± 0.2 5.2 ± 0.3 5.0 ± 0.8 5.4 ± 0.1
APE 400 4.0 ± 0.3 5.7 ± 0.6 7.5 ± 0.7 7.0 ± 1.5 5.3 ± 1.1 5.8 ± 0.8 6.5 ± 0.4
1,000 3.8 ± 0.6 5.0 ± 0.4 5.9 ± 0.5 6.5 ± 0.3 7.3 ± 0.9 7.3 ± 0.8 5.6 ± 0.1
2,000 4.5 ± 0.4 7.6 ± 0.6** 7.7 ± 0.8 9.2 ± 1.0** 8.9 ± 0.9* 8.5 ± 0.6* 6.8 ± 0.6
Morphine 5 3.9 ± 0.2 9.3 ± 0.8*** 12.6 ± 1.2**** 13.1 ± 1.2**** 11.7 ± 1.3**** 10.9 ± 1.0*** 10.7 ± 0.1***

Data indicated as mean ± standard error of the reaction time (sec) of six mice.

Mice were treated with normal saline (10 mL/kg, p.o), APE (400 mg/kg, 1,000 mg/kg, and 2,000 mg/kg, p.o), or morphine (5 mg/kg, i.p). Statistical analyses were performed using one-way ANOVA followed by Dunnett’s multiple comparisons test.

APE: aqueous propolis extract, p.o: orally, i.p: intraperitoneal.

*P < 0.05, and **P < 0.001, ***P < 0.0001, and ****P = 0.0001 represent a significant difference compared to the normal saline group.

Korean J Pain 2024;37:141~150 https://doi.org/10.3344/kjp.23318
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