Fig. 3. Time course of the anti-allodynic and hyperalgesic effects of NaCl administered intrathecally in the rats with mechanical allodynia and thermal and cold hyperalgesia after L5 and L6 spinal nerve ligation. All the behavioral tests were performed at baseline and after 30 minutes, 2 hours, 1 day, and 1 week of administering 0.45%, 0.9%, 2.5%, 5%, 10%, and 20% NaCl intrathecally. (A) VFF test. The withdrawal threshold (grams) to mechanical stimuli is presented on the y-axis, and time on the x-axis. (B) Hot-plate test. The withdrawal latency (seconds) to hot (45°C) stimuli are presented on the y-axis, and time on the x-axis. (C) Cold-plate test. The withdrawal latency (seconds) to cold (4°C) stimuli is presented on the y-axis and time on the x-axis. Vertical bars indicate the standard deviation of the mean. Ten animals were used in each group. Data represent the mean ± standard deviation. VFF: von Frey filaments. aP < 0.05 compared to the baseline. bP < 0.05 compared to the 0.9% NaCl group (0.45% NaCl = open circle; 0.9% NaCl = filled square; 2.5% NaCl = filled up-pointing triangle; 5% NaCl = filled down-pointing triangle; 10% NaCl = open square; 20% NaCl = filled circle).
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