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Fig. 4. The effects of N(gamma)-nitro-L-arginine methyl ester (L-NAME) alone, and prior to L-arginine (L-Arg, 45.92 nmol) microinjections into medial prefrontal cortex (mPFC) on spared nerve injury (SNI) induced mechanical allodynia. Values represent the mean ± SEM (n = 6 animals per group). Microinjection of L-Arg and L-NAME was performed 3 and 6 minutes before mechanical allodynia recordings, sequentially. (A) The paw withdrawal threshold fifty percent (50% PWT) change before and after drugs infusion. Different letters represent significant differences (P < 0.001, two way ANOVA and Bonferroni post hoc tests) for all time-points after microinjections of L-NAME (17.15 nmol) prior to L-Arg (45.92 nmol) compared with only L-Arg (45.92 nmol) treated group in SNI rats. (B) Related area under the curve (AUC) of mechanical allodynia after microinjection of normal saline (NS) and drugs. Different letters represent significant differences (P < 0.001) using one way ANOVA and Tukey’s post hoc tests. LA: L-Arg, SEM: standard error of the mean, ANOVA: analysis of variance.
Korean J Pain 2022;35:413~422
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