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Fig. 1. The microinjection schedule and study timeline used in this study. On day 1, rats underwent spared nerve injury (SNI) surgery. On day 7, medial prefrontal cortex (mPFC) on both sides of the brain was cannulated. Paw withdrawal threshold fifty percentage and locomotor behavior were recorded on the 14th and 20th day, respectively. Intra-mPFC microinjections of L-arginine and antagonists were performed at 6 and 3 minutes before allodynia recording, respectively. Fifteen minutes before, and on 5, 25, 45 and 65 minutes after infusion of drugs mechanical allodynia was evaluated. On day 20, the brains were separated for tip of cannulas verification.
Korean J Pain 2022;35:413~422
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