Fig. 2. (A-C) Expression of toll-like receptor 4 (TLR4) in bilateral spinal dorsal horn of rats with nucleus pulposus (NP). (D) Specificity of antibody is tested by negative control (n = 2). (E-I) Expression of TLR4 in ipsilateral spinal dorsal horn of different groups (*P = 0.001, **P < 0.001, n = 4/group). (J-R) Double immunofluorescence staining of TLR4 (red) with cellular markers (green): ionized calcium binding adaptor molecule-1 (Iba-1, microglia marker), glial fibrillary acidic protein (GFAP, astrocyte marker) and neuronal specific nuclear protein (NeuN, neuronal marker) respectively. (H) The histogram shows Iba-1 positive area of spinal dorsal horn in different groups. Scale bar (A) = 400 μm, Scale bars (B, C) = 200 μm, Scale bars (D-H, J-R) = 100 μm. Error bars indicate standard error of means.
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